Association of Active Human Herpesvirus-6, -7 and Parvovirus

[marlène]

Association of Active Human Herpesvirus-6, -7 and Parvovirus B19
> Infection with Clinical Outcomes in Patients with Myalgic
> Encephalomyelitis/Chronic Fatigue Syndrome.
>
> Chapenko S, Krumina A, Logina I, Rasa S, Chistjakovs M, Sultanova A,
> Viksna L, Murovska M.
> August Kirchenstein Institute of Microbiology and Virology, Riga
> Stradins University, Ratsupites Street 5, LV-1067 Riga, Latvia.
>
> Abstract
>
> Frequency of active human herpesvirus-6, -7 (HHV-6, HHV-7) and
> parvovirus B19 (B19) infection/coinfection and its association with
> clinical course of ME/CFS was evaluated.
>
> 108 ME/CFS patients and 90 practically healthy persons were enrolled
> in the study.
>
> Viral genomic sequences were detected by PCR, virus-specific
> antibodies and cytokine levels-by ELISA, HHV-6 variants-by restriction
> analysis. Active viral infection including concurrent infection was
> found in 64.8% (70/108) of patients and in 13.3% (12/90) of
> practically healthy persons.
>
> Increase in peripheral blood leukocyte DNA HHV-6 load as well as in
> proinflammatory cytokines' levels was detected in patients during
> active viral infection.
>
> Definite relationship was observed between active betaherpesvirus
> infection and subfebrility, lymphadenopathy and malaise after
> exertion, and between active B19 infection and multijoint pain.
> Neuropsychological disturbances were detected in all patients. The
> manifestation of symptoms was of more frequent occurrence in patients
> with concurrent infection.
>
> The high rate of active HHV-6, HHV-7 and B19 infection/coinfection
> with the simultaneous increase in plasma proinflammatory cytokines'
> level as well as the association between active viral infection and
> distinctive types of clinical symptoms shows necessity of simultaneous
> study of these viral infections for identification of possible subsets
> of ME/CFS.
>

Adv Virol. 2012;2012:205085. Epub 2012 Aug 13.
>
> Full paper: http://downloads.hindawi.com/journals/a ... 205085.pdf

[chika]

Bedankt voor de info Marlène.

Ik ben ook positief voor HHV6 en het parvovirus B19.
DML heeft er nooit iets over gezegd... Ik heb me eigenlijk al afgevraagd wat ik ermee aan moet, of er iets mee moet gebeuren met die positieve uitslag.

[annac]

Nou, Marlène, dan gelijk ook volgend artikel hier tegenaan gegooid. Ik las er ergens in, dat vanwege de bekende gedaantewisselingen van virussen als gevolg daarvan medicaties op den duur ook niet meer werken en aldus aangepast dienen te worden.....

Heb die zin uit vergroot.



UC Irvine finds Achilles heel of virus family often implicated in ME/CFS

bron: ProHealth.com
datum: August 29, 2012

For ME/CFS patients especially, this may be an important finding given the
body of research suggesting associations with entero-, coxackie-, and other
picornavirus infections.

________________________

"The primary challenge of antiviral drug resistance may be sidestepped by
targeting a newly identified host cell function required for viral
replication, and not the virus itself."

By discovering how certain viruses use their host cells to replicate,
University of California, Irvine microbiologists have identified a new
approach to the development of universal treatments for viral illnesses
ranging from meningitis, encephalitis, and hepatitis to the common cold.

The UCI researchers, working with Dutch colleagues at Leiden University,
found that certain RNA viruses hijack a key DNA repair activity of human
cells to produce the genetic material necessary for them to multiply.

For many years, scientists have known that viruses rely on functions
provided by their host cells to increase their numbers, but the UCI study -
led by microbiology & molecular genetics professor Bert Semler, PhD - is the
first to identify how the RNA-containing picornaviruses utilize a DNA repair
enzyme to do so.

Study results were published Aug 20 by the Proceedings of the National
Academy of Sciences (PNAS) (See "An RNA virus hijacks an incognito function
of a DNA repair enzyme.")

RNA viruses have ribonucleic acid as their genetic material (rather than
deoxyribonucleic acid, or DNA). Notable human diseases caused by RNA viruses
include:

. SARS,
. Influenza,
. Hepatitis C,
. West Nile fever,
. The common cold,
. Poliomyelitis.

And, as noted, a great deal of research has implicated picornaviruses in
'chronic fatigue syndrome' aka ME/CFS.(1, 2)

The UCI and Dutch researchers examined the picornavirus group of RNA viruses
using biochemical purification methods and confocal microscopy to see how
they co-opt the functions of a cellular DNA repair enzyme called TDP2 to
advance their replication process.

According to Dr. Semler, who directs UCI's Center for Virus Research:

"These findings are significant because all known picornaviruses harbor the
target for this DNA repair enzyme, despite the fact that their genetic
material is made up of RNA rather than DNA.

"Thus, identifying drugs or small molecules that interfere with the
interaction between the virus and TDP2 could result in a broad-spectrum
treatment for picornaviruses."

Sidestepping Problem of Viral Mutation

By targeting a host cell function required for viral replication and not the
virus itself, he added, the primary challenge of antiviral drug resistance
may be sidestepped.

. As part of their survival mechanism, RNA viruses mutate often, and drugs
intended for them usually become ineffective over time.
. HIV, for example, rapidly mutates, necessitating a combination therapy
employing a number of antiviral agents.
. A drug that blocks RNA viruses from hijacking DNA repair enzymes may avoid
these resistance issues.

Dr. Semler has said that his lab now plans to screen mixtures of drug
candidates to find ones that inhibit this process - starting with cells
infected by the human rhinovirus, the predominant cause of the common cold.
(Actually the genus Rhinovirus no longer exists, according to
Picornaviridae.com. The two original human rhinovirus species have been
moved to the genus Enterovirus, meaning virus that infects through a
membrane, as in the gastrointestinal or respiratory tract.)

Ed Note: Increasingly, such screening tasks are aided by databases/libraries
that allow rapid sifting of accumulated information about thousands of
compounds and drugs, as well as by "high-throughput screening" technology
for testing purposes. One such library is reportedly housed at the
University of California, San Francisco, which like other emerging
noncommercial drug libraries has sourced many of its compound samples from
the Johns Hopkins Drug Library. The Hopkins library houses on ice more than
3,000 drugs already approved for clinical use going back a century or more.

Richard Virgen-Slane, Janet Rozovics, Kerry Fitzgerald, Tuan Ngo, Wayne Chou
and Paul Gershon of UCI and Gerbrand van der Heden van Noort and Dmitri
Filippov of Leiden University in the Netherlands participated in the study,
which received support from the American Asthma Foundation and a National
Institutes of Health Public Health Service grant.



Source: Based on University of California, Irvine press release, Aug 20,
2012.
___

1. "Chronic fatigue syndrome is associated with chronic enterovirus
infection of the stomach," by John and Andrew Chia, Journal of Clinical
Pathology, Aug 2007. They found enterovirus infection in stomach biopsies of
135 of 165 (82%) consecutive ME/CFS patients, vs. 20% of controls.

2. "Documented involvement of viruses in ME/CFS," Margaret Williams, Dec
2009.

Pffffff.

Wat een moeilijke materie toch. Wel zinnig om proberen er iets van te blijven begrijpen......

Het valt DML niet kwalijk te nemen dat hij niet altijd in staat is ons dat allemaal op eenvoudige wijze uit te leggen.

[hildeke]

Heel interessant, al versta ik er niet altijd alles van, niet zo medisch aangelegd... Kent er iemand de waarden vanaf welke men positief test?