Op XMRV buzz lees ik al een aantal dagen dat we ons sterk moeten gaan voorbereiden op een hernieuwde discussie over contaminatie. Er is een aantal onderzoekers dat denk bewezen te hebben dat XMRV een labcontaminant is.
Zucht.... maar goed, het is wel heeeel belangrijk dat het goed wordt uitgezocht ......
Waar blijven die resultaten van de blood working group, we hebben ze nodig !!! (waarschijnlijk pas in zomer?)
We hebben ook de Lipkin studie resultaten nodig, maar die wordt hoogst waarschijnlijk pas aan het eind van dit jaar verwacht.
Ik hoop niet dat ondertussen opnieuw door de contaminatiediscussie weer voorbarige conclusies worden getrokken.
anderzeids zou het natuurlijk kunnen dat het idd een contaminant zijn, anowel ik daar sterk mijn twijfels over heb
Ik wacht in spanning af naar de eerste onderzoeken van Lipkin
Nirvana; Oh Me
http://www.youtube.com/watch?v=zgDKHG4K ... re=related
Ik vind dat er sowieso nog veel vragen omtrent xmrv onbeantwoord zijn.
Hoe kan het bijvoorbeeld dat bloedstalen die met verschillende methoden worden getest, verschillende uitslagen geven? Wat heb je dan aan een uitslag?
Nou ja, ik hoop maar dat er een beetje snel uitsluitsel komt.
Waar kan ik nou het beste updates lezen over alle onderzoeken?
Ik ben de draad een beetje kwijt inmiddels
Absence of proof is not proof of absence
Ik zat die eerste link net te bekijken nav je eerste post. Daar staat inderdaad heel veel. Com pleet met forum en chat.
Voorlopig nog genoeg te lezen dus (als mijn hoofd het weer doet).
Absence of proof is not proof of absence
niet al te best betreft CVS.. desalnietemin interesant alleen weer een onderzoeker die de stelling van cvs door xmrv onderuithaalt
(een van de laaste sprekers welke kun je zien door de dia"s te bekijken)
bij de vragen 40 min, word er wel veel over antivirals voor cvs gesproken. vooral over de blogs op internet
http://app2.capitalreach.com/esp1204/se ... odiumVideo
Nirvana; Oh Me
http://www.youtube.com/watch?v=zgDKHG4K ... re=related
http://www.chicagotribune.com/health/ct ... 6823.story
A high-profile scientific paper that gave enormous hope to patients diagnosed with chronic fatigue syndrome, and even prompted some to begin taking potent anti-HIV drugs, has been largely discredited by subsequent research.
Evidence is mounting that a retrovirus called XMRV is not a new human pathogen infecting millions, as was feared, but a laboratory contaminant.
Cancer biologist Robert Silverman, a key researcher at Cleveland Clinic's Lerner Research Institute who worked on studies that linked XMRV to chronic fatigue syndrome and prostate cancer, told the Tribune his lab had stored a cell line known to harbor XMRV and he was working to determine if contamination occurred. Virologists who have examined work by Silverman and others have raised serious questions about contamination, an unfortunate but not unusual mishap in the field.
"I am concerned about lab contamination, despite our best efforts to avoid it," Silverman wrote in an e-mail, adding that similar cell lines "are in many, many labs around the world. Contamination could come from any one of a number of different sites."
A European research team this week reported being unable to find any evidence of XMRV in the blood of people diagnosed with chronic fatigue syndrome and their healthy peers, the latest in a stream of studies in which researchers looking for the retrovirus in the blood of both sick and healthy people have come up empty. Others have reported no evidence of the retrovirus in the blood of patients who were previously found to be XMRV-positive.
The Tribune reported last year that the original research on chronic fatigue syndrome and XMRV had led some patients to get tested for the retrovirus and take anti-retroviral drugs intended to treat HIV, which causes AIDS. The situation highlights the danger in putting too much stock in one scientific study, even one in a prestigious journal. Studies need to be replicated, and early research is often proved wrong.
The original study, published in Science in 2009, was led by retroviral immunologist Judy Mikovits of the private Whittemore Peterson Institute for Neuro-Immune Disease in Reno, Nev. The institute plans to open a clinic that in May would begin treating patients diagnosed with chronic fatigue syndrome and other neuro-immune diseases. Despite the newer research, its leaders strongly deny that contamination could account for their findings.
"It is clearly a human infection," Mikovits, the institute's director of research, told an audience at a January presentation hosted by a California alternative medical practice. "It is clearly circulating through the population as is our fear and your fear."
Scientists say there is no evidence to support her statement.
"Saying that is just inciting fear," said Columbia University virologist Vincent Racaniello.
Mikovits, who once worked at the National Cancer Institute in Frederick, Md., has made increasingly broad statements about XMRV. At the January talk, she showed a slide connecting XMRV to a list of frustrating medical conditions like ALS, Parkinson's disease, multiple sclerosis and dementia. She also linked it to autism. But no published data exist to support those links.
Mikovits also talked about potential treatments, including the powerful anti-retroviral drugs used to treat people who have HIV. These have not been proved safe or effective for people with chronic fatigue syndrome or any of the other conditions listed.
The WPI's director of clinical services, Dr. Jamie Deckoff-Jones, who has chronic fatigue syndrome and has taken anti-retroviral drugs for a year, is using a personal blog to allege a cover-up by researchers seeking to discredit the XMRV link.
"So is there motivation for the cover-up and baseless attacks against Dr. Mikovits?" she wrote in a posting that has been widely circulated on patient forums. "They cannot attack the data because it is impeccable."
WPI President Annette Whittemore, whose daughter has been diagnosed with chronic fatigue syndrome, said in an interview that she thinks politics are at play.
"I thought we were going to solve my daughter's illness or at least fund more significant treatments," said Whittemore, who founded the institute. "I didn't think we would have such political pushback. That was so naive of me."
Whittemore also defended patients trying anti-retrovirals, saying they are safe if used under an experienced doctor's care.
"Patients choose to try these drugs because they are so sick they have lost their entire lives to this illness," she wrote in an e-mail. "As far as I am concerned, they are the pioneers paving the way forward for other sufferers."
In her presentation, Mikovits also described the antiretroviral drugs as "very well tolerated" by patients trying them for chronic fatigue syndrome.
"Very clearly something is going on in the majority of people being treated," she said. "Most don't notice they are taking them."
Physicians who work with HIV patients say antiretroviral drugs can cause significant side effects and that efficacy cannot be determined through anecdotes.
The chasm between the WPI and its supporters and many in the scientific community is emblematic of a new, modern-day dynamic in which patients keep close tabs on the work of researchers and feel empowered to challenge that work and form strong opinions about the quality of it.
Early on, many in the online chronic fatigue community threw their support behind WPI, believing strongly that XMRV was the cause of their illness. More than 1,000 people have paid for non-FDA-approved XMRV blood tests from a commercial lab associated with WPI and headed by Whittemore's husband, Harvey, according to state records. The tests range from $249 to $450, according to the lab website.
Patients' ardent support for XMRV as a cause has continued as other research teams have failed to find any evidence that it was true.
On one patient message board, a commenter wrote in February about not only having contributed multiple times to WPI but also having "sent letters, e-mails, tried to contribute ideas, talked with both Judy M. and Annette, considered whether there's an opportunity for venture capital funding, am willing to protest, knit a pair of socks, etc."
This month, 4,000 scientists and clinicians gathered in Boston for a retroviral conference that included 10 presentations offering evidence that XMRV is a lab contaminant. Mikovits did not attend.
Retrovirologist Jonathan Stoye, who co-wrote a supportive commentary that accompanied Mikovits' original study linking chronic fatigue syndrome and XMRV, said he has since changed his mind.
"I think there are serious problems," said Stoye, who works at MRC National Institute for Medical Research in London.
His co-author, John Coffin, a retrovirologist at Tufts University, agreed the evidence for a link between XMRV and human disease had been seriously weakened.
"I think most people are reasonably convinced that there is not much left anymore," Coffin said. But, he said, "I don't think everything has been nailed down."
Coffin began harboring doubts about Mikovits' study as negative evidence piled up and after he, researcher Vinay Pathak at the National Cancer Institute and their colleagues found what they believe to be the parent viruses of XMRV.
The viruses, according to research Pathak presented at the Boston conference, recombined in a cell line called 22RV1 to create a new retrovirus — XMRV — sometime in the 1990s. The work is in the publication process.
That widely used cell line had been stored in Silverman's lab before he found evidence of the retrovirus in the prostate tissue of patients with a form of prostate cancer.
"22RV1 cells were once previously (more than a year earlier) grown in my lab but were being stored in a liquid nitrogen freezer at the time, and not the same freezer used to store prostate tissues," Silverman wrote in an e-mail. "At the time it was unknown that 22RV1 cells were infected with XMRV."
In the field of virology, contamination has sometimes been mistaken for real results. Greg Towers, a virologist from University College London, notes that the technology used is so sensitive that it takes only one molecule of genetic material to contaminate a sample.
Scientists have been reluctant to shut the door completely on the possibility that XMRV really is tied to human disease. Some questions remain unanswered, said Racaniello, of Columbia University. "I don't think it is time to put a lid on it," he said. "You have to carry the whole thing to its conclusion."
The XMRV story is, to Racaniello, an amazing opportunity for people to watch how science works in real time.
"It is like Watergate," he said. "You saw the Constitution work. You think, oh my gosh, it works! And this is science working. Science determines the truth. … It always sorts it out in the end."
No association of Xenotropic Murine Leukemia Virus-related virus with prostate cancer or chronic fatigue syndrome in Japan
Rika A Furuta , Takayuki Miyazawa , Takeki Sugiyama , Hirohiko Kuratsune , Yasuhiro Ikeda , Eiji Sato , Naoko Misawa , Yasuhito Nakatomi , Ryuta Sakuma , Kazuta Yasui , Kouzi Yamaguti and Fumiya Hirayama
Retrovirology 2011, 8:20doi:10.1186/1742-4690-8-20
Published: 17 March 2011
The involvement of xenotropic murine leukemia virus-related virus (XMRV) in prostate cancer (PC) and chronic fatigue syndrome (CFS) is disputed as its reported prevalence ranges from 0% to 25% in PC cases and from 0% to more than 80% in CFS cases. To evaluate the risk of XMRV infection during blood transfusion in Japan, we screened three populations--healthy donors (n = 500), patients with PC (n = 67), and patients with CFS (n = 100)--for antibodies against XMRV proteins in freshly collected blood samples. We also examined blood samples of viral antibody-positive patients with PC and all (both antibody-positive and antibody-negative) patients with CFS for XMRV DNA.
Antibody screening by immunoblot analysis showed that a fraction of the cases (1.6-3.0%) possessed anti-Gag antibodies regardless of their gender or disease condition. Most of these antibodies were highly specific to XMRV Gag capsid protein, but none of the individuals in the three tested populations retained strong antibody responses to multiple XMRV proteins. In the viral antibody-positive PC patients, we occasionally detected XMRV genes in plasma and peripheral blood mononuclear cells but failed to isolate an infectious or full-length XMRV. Further, all CFS patients tested negative for XMRV DNA in peripheral blood mononuclear cells.
Our data show no solid evidence of XMRV infection in any of the three populations tested, implying that there is no association between the onset of PC or CFS and XMRV infection in Japan. However, the lack of adequate human specimens as a positive control in Ab screening and the limited sample size do not allow us to draw a firm conclusion.
http://www.ncbi.nlm.nih.gov/pubmed/2140 ... t=Abstract
PLoS One. 2011 Mar 9;6(3):e17592.
Investigation into the Presence of and Serological Response to XMRV in CFS Patients.
Erlwein O, Robinson MJ, Kaye S, Wills G, Izui S, Wessely S, Weber J, Cleare A, Collier D, McClure MO.
Jefferiss Research Trust Laboratories, Section of Infectious Diseases, Wright-Fleming Institute, Faculty of Medicine, Imperial College London, London, United Kingdom.
The novel human gammaretrovirus xenotropic murine leukemia virus-related virus (XMRV), originally described in prostate cancer, has also been implicated in chronic fatigue syndrome (CFS). When later reports failed to confirm the link to CFS, they were often criticised for not using the conditions described in the original study. Here, we revisit our patient cohort to investigate the XMRV status in those patients by means of the original PCR protocol which linked the virus to CFS. In addition, sera from our CFS patients were assayed for the presence of xenotropic virus envelope protein, as well as a serological response to it. The results further strengthen our contention that there is no evidence for an association of XMRV with CFS, at least in the UK.
http://www.jurology.com/article/S0022-5 ... 0/fulltext
weliswaar in lage aantallen,en bij prostaatkanker, maar als het virus helemaal niet zo bestaan en een pure contaminant zou zijn (zoals coffin et al nu roepen) dan zou je, in een zuiver lab, alleen maar 0/0 studies moeten krijgen...
afwachten wat mikovits op het eind van de maand te vertellen heeft....( 29 maart)
Met de nadruk op de laatste 5 woorden ... Stel dat XMRV toch de oorzaak is van ME, dan zijn mensen in de UK er dus immuun voor.The results further strengthen our contention that there is no evidence for an association of XMRV with CFS, at least in the UK.
Niet toevallig dat Wesseley mede-auteur is van dit artikel.
Het is makkelijker een atoom te splitsen dan een vooroordeel. - Einstein
Be critical and use your own judgement when using this information. - Marlène R.
Zei van der Meer ook niet hetzelfde, dat XMRV alleen in de USA voorkwam. Onze eigen poll toont aan dat dit niet zo is.
Neemt niet weg dat het belangrijk is om uit te zoeken hoe dat nu zit met waarom de een wel iets vindt en de ander niet. Laten we hopen dat de Blood Workig Group met het verlossende woord komt...